Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
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Examination of the Mouse Embryo by Micro-CT
Noriko HIRAIWAMutsumi ISHIMOTOHiroshi YASUE
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JOURNALS FREE ACCESS

2013 Volume 62 Issue 1 Pages 57-61

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Abstract

Micro X-ray computed tomography (micro-CT) is widely used in preclinical studies of small animals. However, due to the low soft tissue contrast, segmentation of soft tissues in the micro-CT image is a challenging problem. To gain a better understanding of the macroscopic anatomy of the mouse embryo, 3 fixation methods and 3 metal stainings were examined for micro-CT using C57BL/6J mouse embryos in the present study. The examination demonstrated that 1% acetic acid/95% ethanol fixative together with zinc staining provided a high contrast micro-CT image, enabling the segmentation of soft tissues. Then, using this condition, the macroscopic embryo structure of the nude mouse was examined, revealing lack of a thymus. It appears that micro-CT with the fixation and staining condition devised in the present study could be a powerful tool in detecting the effects of various mutations at embryonic stages.

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© 2013 Japanese Association for Laboratory Animal Science
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