抄録
1. Time required for rearing and experiments of germfree mice were measured, with respect to 5 operators. Operators consist of 2 veterans (A, B) having studied on germf ree animals since more than 4 years, and 3 beginners (C, D, E) just having finished a short training course on germf ree technique. Results are summarized in Table 1. On the operation time, there was no significant difference between individuals. As shown in the table, 3271 sec./isolator were required for assembling vinyl isolator, 1371 sec. for sterilization of the inside of chamber with 2 % peracetic acid, 1233 sec. for the transfer of materials through vinyl sleeve, 101 sec. for the feeding, breeding, cleaning and recording, and 1078 sec. for cleaning the isolator after experiment.
2. Using 24 isolators, 93 germf ree mice were bred and 78 were used for experiments from April 10 to July 18 in 1971 in our laboratory. Daily total times required for germfree rearing and experiments are shown in Figure 1. For this period of 100 days, 52.9 min./day (mean) were spent for germfree animals.
