Fisheries science
Print ISSN : 0919-9268
There Are 4 Introns in the Gene Coding the DNA-binding Protein HCc of Crypthecodinium cohnii (Dinophyceae)
Takeshi Yoshikawa, Aritsune Uchida, Yuzaburo Ishida
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1996 年 62 巻 2 号 p. 204-209

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抄録
HCc is a major basic nuclear protein of Crypthecodinium cohnii (Dinophyceae). Inorder to elucidate the gene organization of HCcl gene, it was amplified by PCR methods using the total DNA as atemplate and the nucleotide sequences of HCcl gene and its flanking regions wereestablished. Theresults were as follows: [1] HCcl gene was composed of multiple copies and arranged in tandem; [2] itcontained 4 introns lacking the GT-AG consensus sequences, which are conserved inother eukaryoticintrons; and [3] eukaryotic promoter consensus sequences such as TATA box or CAAT box were not observed in the flanking regions of HCcl gene. An interesting pointis whether such atypical-eukaryotic features of the HCcl gene organization are common in dinoflagellates or not.
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© The Japanese Society of Fisheries Science
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