structural Characterization of the Carbohydrate Chains form the Skin Mucus Sialoglycoprotein of the Stingray Dasyatis akajei

抄録

Carbohydrate chains were liberated from stingray skin mucus sialoglycoprotein (SGP) in the form of oligosaccharide alditols by alkaline borohydride treatment. A major oligosaccharide alditol and more than 4 minor ones were detected. The major oligosaccharide alditol (37mg) was isolated from 100mg of SGP. Using methylation analysis, the major oligosaccharide alditol was identified to be NeuAcα2→6GalNAc-ol. Based on the experimental results, an average SGP molecule was estimated to contain more than 400 sialic acid-containing oligosaccharide chains such as NeuAcα2→6GalNAc which are bound to protein core through GalNAc residues.