抄録
The dietary value of rotifers Brachionus rotundiformis cultured with Synechocystis sp. SY-4 (S-rotifer) or rotifers secondary-cultured with Nannochloropsis oculata for 24h after culturing with Synechocystis sp. SY-4 (SN-rotifer) was compared with those cultured with N. oculata (N-rotifer) by feeding these rotifers to the larvae of red sea bream Pagrus major and Japanese flounder Paralichthys olivaceus. The growth and survival rates of those fishes fed on SN-rotifers were better than those fed on S-and N-rotifers. The proximate composition of rotifers was significantly different depending on the algal species used for the culture and the mode of culture. The difference in growth and survival rates among the fish larvae might be due to the difference in the content of n-3 highly unsaturated fatty acid (n-3 HUFA) in the feeds. The percentages of n-3 HUFA in the total fatty acids of S-, SN-, and Nrotifers were 10.3, 23.6, and 20.8%, respectively. The amount of n-3 HUFA in S-rotifer was not enough (0.2%/rotifer dry-weight) as feed for the larvae, but the amount was enhanced by secondaryculture with N. oculata (1.0%/rotifer dry-weight) which was higher than that in N-rotifers (0.5%/rotifer dry-weight).
