Fisheries science
Print ISSN : 0919-9268
Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus
TAKUMA SUGAYAMINORU IKEDAHIDESHI MORINOBUHIKO TANIGUCHI
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2002 年 68 巻 2 号 p. 299-305

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The allelic inheritance mode of microsatellite DNA markers was examined using seven copulated wild females and their offspring. Five microsatellite loci, CSPJ002*, CSPJ010*, CSPJ012*, CSPJ014*, and CSPJ015*, were used in the study. At almost all family/locus combinations, one sire was determined and distributions of genotypes in offspring were consistent with the Mendelian segregation ratio. Distributions of genotypes were consistent with the ratio after assuming a null allele at some loci. Consequently, the alleles of CSPJ002* and CSPJ012* were inherited following the Mendelian inheritance mode in every family; however, the null allele was expected in CSPJ010*, CSPJ014*, and CSPJ015* in some families. Thus, these loci should be used carefully in population genetic analysis, but siblings could be detected in the dendrograms based on unweighted pair-group method using arithmetic averages (UPMGA).
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