Fisheries science
Print ISSN : 0919-9268
Homing endonuclease and mobile intron in the novel hyperthermophilic archaeon Aeropyrum pernix isolated from marine hydrothermal vent
YOSHIHIKO SAKONORIMICHI NOMURAYAYOI MORINAGAARITSUNE UCHIDA
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2002 年 68 巻 sup2 号 p. 1173-1176

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Marine hyperthermophilic microorganisms are valuable resources for thermostable enzymes which are well suited for biotechnological applications. A novel DNA endonuclease I-Ape I was found from the hyperthermophilic archaeon Aeropyrum pernix and characterized. Of particular interest is that I-Ape I is encoded in the optional intron Iα within the 16 S rRNA gene. Probably, I-Ape I confer mobility to the host intron Iα, like group I intron homing endonucleases from eukaryotes and bacteriophages. Also, this rarecutting enzyme is a useful tool for genome mapping.
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© The Japanese Society of Fisheries Science
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