Bulletin of Society of Japan Science Teaching
Online ISSN : 2433-0140
Print ISSN : 0389-9039
The Study on the Development of Students' Biological Experiments Using Chiken Embryos - To Observe Developmental Process, Somatic Division and Chromosome -
Kiyoshi Nonoyama
Author information
JOURNAL FREE ACCESS

1984 Volume 24 Issue 3 Pages 21-29

Details
Abstract

The author has developed an effective laboratory methods for observing developmental processes and somatic divisions and for making chromosome specimens using chicken embryo. These newly developed methods, he believes, enable students to understand complex life phenomena synthetically. "Human Biology" is very important for the student but it is difficult to make experimental materials from the human body. "Chicken Biology" can solve this difficulty and give us several superior methods which could not get from amphibian or other lower animals. Moreover the corpuscle used here can play a roll to combine physiological phenomena with a developmental process and a cell division. The methods described here are as follows; (1) Take off a small piece of eggshell and membrane just over the embryo of a chicken egg after two days from warming, and make a small window to observe the embryo. Covering the window with transparent cellophane tape, continue to warm the egg. In 48 hours after opening eggshell about 50% of the eggs were able to survive. Students can observe dramatic changes of the morphogenesis and differentiation of the embryo through the window. (2) For the observation of the somatic division, the corpuscle of the chicken embryo can be used instead of the root of plants used before. The bloods of the fourth day chicken embryo was smeared on the slide glass and stained with Feulgen's nuclear reaction and Giemsa. The ratio of corpuscle in division of the fourth day embryo was 3.4%. (3) A chromosome specimen can be made from the corpuscle of chicken embryo. Open a window on the eggshell of the fourth day embryo, add colchicine and continue to warm. In one hour after adding colchicine, take off the blood from the embryo and conduct a hypotonic and fixation. Put floating corpuscle on the little amount of fixation solution upon the slide glass and dry it in the flame. Staining it with Feulgen's nuclear reaction, it can be used for observation. This method does not require a cultivation nor skillful squash method. It takes about two hours for this experiment including a colchicine treatment.

Content from these authors
© 1984 Society of Japan Science Teaching
Previous article Next article
feedback
Top