生活組織影響下に認められたるSulfonamid-劑の變化に關する考察

抄録

To make clear the mode of the germicidal action of the Sulfonamides these experiments were carried out using tissue culture.
The heart tissues of the chicken embryos were cultivated after the methode described byCarreland the following day the desired amount of drug and staphylococci were added to the tissue culture. This was stored for 4 hours at 38°C, after which the desired amount of the culture medium was withdrawn and mixed thoroughly in blood agar to form blood agar plate. After 16 hours of incubation at 37°C, the number of colonies per plate was counted. Simultaneously, the amount of the conjugated drug in the culture medium was determined after the method ofEastman. The results are summarized as follows : 1) Sulfonamides are oxidized in tissue culture and developed a powerful bacteriostatic activity. 2) The drug started to affect the growth and multiplication of staphylococcus aureus in the concentration of 0, 0016 %, while in 0, 16% it caused moderate suppression of them and in higher concentration as 0, 16 % or over the action of the drug was developed markedly. 3) The effect of the drug on staphylococcus aureus increased with the increase in temperature and this may be explained on basis that the higher temperatures produced relative increase in the conjugation of the drug. 4) Slight suppression on the growth and multiplication of the organisms were observed after 2 hours contact with the drug, moderate suppression after 4 hours and marked suppression after 6 hours such that at 12 hours the drug acted almost bactericidal. The amount of the conjugated drug increases up to 4 hours, after which it remains almost constant. 5) Sulfonamides reduced the amount of hemolysis produced around the colony. This effect is not due to the neutralization of toxins but it is secondary to bacteriostasis.[Cf. original (Japanese) p. 366.]