組織培養法に依る各種Sulfonamid-劑の作用比較

抄録

Fibroblasts were cultured from the hearts of 8-9 day old chicken embryos using the coverglass method, in a medium composed of an equal quantity of plasma and embryonic extract. The action of the sulfonamides differed according to the concentration of the drug although a very dilute solution (%) of the drug had no appreciable effect on the growth of the fibroblastes, the growth was found to be accelerated in dilute solutions (%), the intensity being proportional to the increase in the concentration, but to a certain degree and then the growth decreased with the increase in the concentration. Higher concentrations suppsessed the growth in proportion to the increase in the concentration till the growth was completely checked. It was of interest to note that in the concentrations lying between dilute and strong as well as in the very dilute concentrations, the growth of the culture was neither promoted nor suppressed.
When the growth of the cells were enhanced by a dilute solution of the drug, the cells, especially at the periphery of the culture, showed better development than the center and were arranged regularly and more closely together and closely interrelated to the protoplasmic process. When the growth of the culture was suppressed by the stronger solutions of the drug, the degenerative process gradually extended from the external to the internal zones. The processes involved in the degenerative process were 1) the reticular arrangement became gradually irregular and scattered ; 2) very fine fat granules gradually increased from the proximal pole of the cell to the center in relation to the degeree of degenerative process ;. and 3) the nucleus gradually showed karyolysis and became pycnotic and the cytoplasm became vacuolated, when the growth of the cultute was strongly suppressed, the degenerated internal zone consisted of prickly projections from the mother tissue.
The relationship between the chemical construction of the derivatives and its action were as follows : 1) sulfanilyl-dimethyl-sulfonamide, sulfapyridine and Sulfathiazole were the most toxic, Sulfanilyl-monomethyl-sulfonamide and Sulfanilyl-sulfanilamide being slightly less, and 4'-sulfanily1-2, 4-diamino-azobenzene-sulfanilamide, di-4 (4'-aminophenyl-sulfonamide-phenyl-sulfanilurate and para-aminophenyl-sulfanilacetamide were still less toxic than those listed above, in the order named : 2) Drugs consisting of 2 bases showed the toxicity to be more proportional to the number of CH, i. e., sulfanilyl-dimethyl-sulfasulfanilamide was the most toxic and sulfanilyl-monomethyl-sulfanilamide was slightly more toxic than sulfanilyl-sulfanilamide : and 3) drugs composed of one base, such as sulfapyridine and sulfathiazole were more toxic than others and paraaminophenyl-sulfanilacetamide was less toxic than sulfanilamide. [Cf. Original (japanese) P. 352.]