抄録
On 100 biopsy specimens obtained endoscopcallly the distribution of mucopolysaccharide in the duodenal mucosa and the mucus layer covering on the surface of the mucosa, its biochemical analysis histoimmunological analysis, and mechanism of its secretion were researched. The results are as follows: 1) The mucous substance could be observed endo-scopicaly by spreading 0.2% Azure A. 2) The duodenal mucosa was rich with the PAS-positive substance, as well as the gastric mucosa, and acidic mucopolysaccharide of the Alcian bluepositive was more in the former. The covering mucous layer of the duodenum was composed of a double layer construction of the PAS-positive layer and the Alcian blue-positives, but it was thinner than the gastric one. In the duodenal crypt, Alcian blue-positive (acid mucopolysaccharide) was more rich than in the stomach. 3) According to 3H-glucose and 35SO4 autoradiography, acidic and neutral mucopolysaccharides were observed in the cylindrical epithelial cells in which the presence of mucopolysaccharide had not been proved histochemically. Thus, the cylindrical epithelil cell group appeared to be the cell group secreting the PAS-positive substance in the mucus layer covering on the duodenal mucosa. Brunner's glands were similar : many goblet cells in the crypt were Alcian blue-positive, and the Alcian blue-positive substance in the mucus layer appeared to be mostly derived from the goblet cells. 4) Hexosamine was examined for observing the change of mucopolysaccharide in the intestinal mucosa and antral mucosa of the stomach. Muco-Polysaccharide level of normal antral mucosa were 2.114 mg/g and normal duo-denal mucosa were 1.994 mg/g. 5) Considering that secretory component (sc) in the digestive tract was glycoprotein, we discussed in the each stage immunohistologically about variation of the distri-bution of sc, and s-IgA. IgAcontaining cells were proved to increase, and sc decrease in the active stage ulcer. As to the variation of the mucus substance in the duo-denal ulcer, the content in the mucosa and the covering mucus layer will be further pursued in the aspects of various factors, the mucus barrier and secretory immuno-globulin will have to be disscussed in the future.
