Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
ISSN-L : 1341-7568
Short communication
Effective breakage of phage λ DNA by shearing with ceramic-coated needle of syringe
Hachiro InokuchiYukio Inokuti
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2005 年 80 巻 1 号 p. 79-82

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The loss of biological activity of phage λ DNA was much greater when the DNA was sheared using a ceramic-coated needle attached to a syringe compared with a conventional stainless steel needle. Inactivation of the biological activity was due to breakage at the middle of the molecule. The thickness of the ceramic-coating was a crucial factor for the breakage. Because approximately the same level of inactivation was observed with a non-coated needle as with thin glass and quartz tubes, it was concluded that the unknown characteristic(s) of the silicon nitride (SiNx) coating itself resulted in the effective breakage of λ DNA molecules by shearing force.

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© 2005 by The Genetics Society of Japan
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