Cloning of P-elements from P- and Q- strains in natural populations of Drosophila melanogaster in Japan

抄録

We have cloned and characterized several P transposable elements from P and Q strains extracted from natural populations of Drosophila melanogaster in Japan. Structures of these clones were investigated by the techniques of blot hybridization, restriction mapping, and observation of heteroduplex by electron microscope. No intact P element such as pπ 25.1 was found among 11 clones from a P strain and 10 clones from Q-strains. A typical Q-strain (WY-113) judging from the results of genetical crosses did not carry any DNA segment homologous to pπ 25.1, indicating the complex nature of Q-strains.