日本草地学会誌
Online ISSN : 2188-6555
Print ISSN : 0447-5933
ISSN-L : 0447-5933
短報
蛍光 in situ ハイブリダイゼーション(FISH)法による東北地方で収集されたリードカナリーグラス(Phalaris arundinacea L.)と外来リードカナリーグラスにおけるrDNAの物理的マッピング
木村 健智上山 泰史久保田 明人藤森 雅博高原 美規秋山 征夫
著者情報
ジャーナル オープンアクセス

2014 年 60 巻 1 号 p. 55-58

詳細
抄録
Reed canarygrass (Phalaris arundinacea L.) has been recognized as a major invasive plant in the US in recent years. In Japan, the differences between the native and invasive genotypes are unclear. To identify these differences, chromosomal analysis using fluorescence in situ hybridization (FISH) with 5S/45S rDNA probes was carried out on 7 populations of putative native Japanese P. arundinacea and 3 exotic P. arundinacea. The results showed that all populations were tetraploids (2n=4x=28). The 45S rDNA were mapped on 4 sites corresponding with the ploidy level in all populations. On the other hand, the numbers of 5S rDNA sites differed among the populations. Moreover, the 5S rDNA sites differed among individuals even within the same populations. Thus, the chromosomal characteristics could not ensure that the putative native Japanese P. arundinacea are the native.
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© 2014 日本草地学会
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