特集記事
多分散・多成分溶液中の生体高分子の構造解析のための小角散乱と各種成分分離手法の複合的アプローチ
2022 年 32 巻 1 号 p. 16-20
詳細
2022 年 32 巻 1 号 p. 16-20
Small-angle X-ray/neutron scatterings (SAXS/SANS; collectively called SAS) offer the structure of a biomacromolecule under near a physiological condition. To reveal a structure of a single biomacromolecule with SAS, it is indispensable to acquire the scattering profile which does not include the contribution of aggregates and other components. In this manuscript, we introduce two state-of-the art methods, Size Exclusion Chromatography-SAS (SEC-SAS) and Analytical UltraCentrifugation-SAS (AUC-SAS), for realizing the selective observation of the concerned component in polydisperse and/or multi-component biomacromolecular solutions.
