抄録
Tissue engineered heart valves based on acellular tissue have been studied to have more durability and bio-functionality with growth potential and less immunogenicity. Whereas they have still several problems to be solved such as complete cell removal and transfer of unknown animal related infectious diseases. In this paper, our novel tissue processing for decellularization using ultrahigh pressure for the safe tissue transplantation was reported. Porcine cardiac tissues were isolated and treated by a cold isostatic pressing for a disruption of donor cells. The cell debris was then washed out by washing solution at 4°C. The tissues treated were completely cell free when they were applied to 980 MPa for 10 min. There was no porcine endogeneous retrovirus detected. There were no significant changes in biomechanical properties of the breaking strength and elastic modulus. The acellular grafts of pulmonary valve were transplanted to allogeneic miniature pigs. The explanted grafts showed remarkable cell infiltration and endothelialization. This processing may provide more durable and safe scaffold for the regenerative tissue transplantation.
