抄録
The fluorescence spectra of 6-propionyl-2-(dimethyamino)-naphthalene (Prodan) in the bilayer membrane of an ether-linked phospholipid, dihexadecylphosphatidylcholine (DHPC), were observed as a function of temperature and pressure. Previous results for Prodan in the bilayer membrane of an ester-linked lipid, dipalmitoylphosphatidyl-choline (DPPC), were compared with the present results. A great difference in the fluorescence spectrum shape was found in the ripple gel phase of the DHPC and DPPC bilayers. The location of the Prodan molecule in the bilayer was speculated on the basis of the maximum emission wavelength. The ratio of fluorescence intensity of Prodan at two wavelengths was useful for the determination of bilayer phase transition, especially the pressure-induced interdigitation.
