Steroid hormones (SH) play an important role in living bodies such as control of homeostasis, immunogenicreaction, and ageing/development for health longevity. Thus, the similar SH effect of phytochemicals hasindicated human promoting heath using these supplements. Previous study showed that diosgenin (similar SHchemical) from Dioscorea esculenta have improvement effect of motor ability. On the other hand, thesebiological monitoring and investigation of SH and diosgenin are insufficient. In the development phase, SHprofiling from various types of studies such as in vitro, in vivo, and clinical studies have to be analyzed. Here,in this study, we tried to develop the simple and simultaneous analytical method for 17 series of SHs usingliquid chromatography tandem mass spectrometry (LC-MS/MS). The method validated in vitro, in vivo,clinical model samples and applied to diosgenin supplied rat serum analysis.
