培養神経回路網の形成過程における自発性神経活動と培養時のブドウ糖濃度の関係性

抄録

Cultured rat hippocampal network on a multi electrodes array (MEA) dish is useful for analyzing the relationships between electrical activity and culture condition during developing the networks. Seeded neurons autonomously form a complex network on a MEA dish and autonomous electrical activity is often observed without any inputs from outer world. Autonomous activity is considered to reflect internal states of the neuronal network. We elucidated that there are a particular relationship between autonomous activity and glucose concentration of culture medium at 7.5 mM, 17.5 mM and 30 mM during developing neuronal network. In the case of 17.5 mM glucose concentration, We confirmed that the frequency of autonomous electrical spikes increased accompany with culture days. In the case of 30 mM glucose concentration, trend of autonomous activity was similar to the case of 17.5 mM. Thus, in the case of 30 mM glucose concentration, glucose neurotoxicity was not obviously observed. Also in the case of 7.5 mM glucose concentration, frequency of autonomous electrical spikes similarly increased during culture days, however, the frequency was significantly low comparing width the case of 17.5 mM glucose concentration. In addition, the frequency of electrical spikes in the condition of 7.5 mM glucose concentration and the initial density of 7800 cells / mm² was similar to the frequency of the spikes in neuronal networks cultured at the initial density of 2600 cells / mm² or 3900 cells / mm² in 17.5 mM glucose concentration. These results show that neurons adjust the culture size and electrical activity to the glucose concentration of culture medium during developing a neuronal network.