We have developed a 3D Lab-on-a-CD immunosensor based on a competitive/non-competitive assay for small endocrine disruptors and biomolecules. To improve the sensitivity of the 3D Lab-on-a-CD immunosensor, an immobilizing method of capturing-type antibody on the sensor surface was investigated. The method is based on the introduction of a highly reactive amino group to a P(MMA/CEMA) copolymer surface having chlorine, and utilizing a biotin-streptavidin system. The functionality of the 3D Lab-on-a-CD immunosensor was evaluated by measuring mouse IgG. The detection limit was found to be 3 f g/assay (3×10-12 g/assay). After further modification, the 3D Lab-on-a-CD immunosensor, having some characteristics such as a substrate with highly integrated antibodies, is expected to be applicable for highly sensitive immunoassay.
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