神経細胞表面におけるAMPA型グルタミン酸受容体分子の光捕捉過程

抄録

AMPA-type glutamate receptor (AMPAR) is one of neurotransmitter receptors at excitatory synapses in neurons. For realizing the artificial control of synaptic transmission, we have applied optical trapping of quantum-dot (QD) conjugated AMPARs on neurons. Here, we evaluate optical trapping dynamics of QD-AMPARs on neurons by single particle tracking (SPT) analysis. The diffusion coefficient derived from mean square displacement (MSD) at laser focus of QD-AMPARs on neurons decreased with the laser irradiation and the culturing days, which suggests that the molecular dynamics of QD-AMPARs are constrained at the focal spot due to optical trapping forces.