電気生理学的モデルを用いた海馬神経調節物質の作用メカニズム

抄録

The mechanism of neuromodulation with serotonin was electrophysiologically revealed during excitatory neurotransmission in the hippocampus by use of the stimulation-recording method. Stimulating electrodes were positioned at the granule layer, and recording electrodes were set at the CA 3 layer in the hippocampal slice preparation of rats. Presynaptic neurons stimulated with single or double pulse train induced excitatory postsynaptic potentials (EPSP). EPSP was recorded and evaluated in the CA 3 injected with serotonin (0, 10, 20, 50 μ M) by means of the wave analyzing program. Firstly, results of the single pulse stimulation show that serotonin pharmacologically reduces EPSP in the hippocampal CA 3 neurons. Secondly, evaluated EPSP responses to the double pulse stimulation are applied to an electrophysiological model. Consequently, it is suggested that serotonin inhibits the release of calcium dependent synaptic vesicles as a neuromodulating mechanism.