電気学会論文誌E(センサ・マイクロマシン部門誌)
Online ISSN : 1347-5525
Print ISSN : 1341-8939
ISSN-L : 1341-8939
特集論文
体内毒性試験へ向けたオンチップ小腸—肝臓由来細胞共培養システムの開発
木村 啓志中山 秀謹山本 貴富喜酒井 康行藤井 輝夫
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2009 年 129 巻 8 号 p. 252-258

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We developed a chip-based coculture system for cytotoxicity test, as our continuous effort to develop a multi-functional micro culture device realized by integration of fluidic control. The culture zone in the device was divided into two compartments separated by a microporous membrane through which substances in culture medium can freely come-and-go to induce the mutual interactions between the cells cultured at each compartment. In this work, it was examined that 1) coculture and 2) cytotoxicity model through oral intake, using Caco-2 and Hep G2 cell as a model cell of small intestine and liver respectively. As a result of test 1), Hep G2 cells cocultured with Caco-2 show same albumin secretion activity as the one not cocultured with Caco-2 cells. As a result of test 2), The cytotoxicity of caffeine and paraquat on Hep G2 cells was successfully measured with and without association of a selective chemical barrier function of Caco-2 cells.

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