On-chip Glucose Sensor for Online Measurement of Cell Activities

Abstract

In this study, we developed a cell-based microfluidic device, which is integrated micro-electrochemical sensors, for online measurement of cellular glucose consumption under perfusion culture. Glucose sensors are placed at inlet and outlet of a cell culture chamber. The cellular glucose consumption is measured by comparing concentrations of dissolved glucose in culture medium before and after contact with the cells. As result of our preliminary test, online measurement of the difference of glucose concentration by cells succeeded using the device. We believe that the cell-based microfluidic device can be applied to bio- and medical science.