Development of a High-Throughput DNA Fiber Analysis Device Using MEMS Technology

Abstract

In this study, we propose a simple observation method of the shape and molecular orientation of the chromosomes extracted from cells for a sample-to-analysis system in clinical diagnosis. The proposed technique is composed of total preparation technique such as cell immobilization, chromosomes extraction, stretching, suspension and analysis using a disposable microchip controlled by centrifugal force only. It is experimentally confirmed that the chip having two kinds of microstructures arranged concentrically on a chip immobilizes cells and stretches chromosomes extracted from the immobilized cells.