Microsized cell-laden hydrogel blocks are attractive cellular building blocks for in vitro reconstruction of 3D macroscopic tissues in bottom-up tissue engineering techniques. This review introduces microfabrication and microfluidic formation methods of the cell-laden hydrogel blocks, and manipulation techniques to assemble them for construction of the 3D macroscopic tissues. Moreover, we show applicability of the cell-laden hydrogel blocks and the 3D macroscopic tissues to biological research and medical treatments.
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