バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価

抄録

Human-derived acetone vapor could be utilized in clinical practice. In this paper, we prepared and demonstrated secondary alcohol dehydrogenase (S-ADH) immobilized mesh toward acetone vapor imaging. Immobilized S-ADH activity was evaluated by autofluorescence of reduced nicotinamide adenine dinucleotide which is consumed in S-ADH catalytic reaction with acetone. As a result, a glutaraldehyde crosslink method showed higher S-ADH activity and allowed fluorometric imaging of acetone vapor without distortions.