IEEJ Transactions on Sensors and Micromachines
Online ISSN : 1347-5525
Print ISSN : 1341-8939
ISSN-L : 1341-8939
Special Issue Paper
Evaluating Morphology and Functionality of On-Chip Vascular Network Derived from Human Umbilical Vein Endothelial Cells (HUVECs) during Long-Term Culture
Atsuya KitadaManeesha ShajiTaiga IrisaKazuya FujimotoStanislav L. KarstenRyuji Yokokawa
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2023 Volume 143 Issue 5 Pages 82-88


Oxygen and nutrient supply provided via the vascular system is essential for organ development and tissue metabolism. Recent advances in stem cell technologies have led to the development of complex 3D tissue culture systems. However, direct supply of nutrients and oxygen by simple diffusion fails to reach inner areas resulting in impaired maturity of these complex tissues over time. In an attempt to alleviate this problem, 3D tissues are integrated with vascular networks (VNs) on a chip to ensure appropriate oxygen and nutrient supply. Such an in vitro vascular system must maintain a stable, robust, and perfusable network without degradation during entire culture period that may easily exceed several weeks. Current protocols for generation of VNs provide no information on the changes in these parameters in the long term. Here, using human umbilical vein endothelial cells, we generated a vascular network on a microfluidic chip and evaluated its morphology and function for 35 days. We demonstrate network stability, preservation of lumen structure and robust perfusability with negligible increase in permeability over time.

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© 2023 by the Institute of Electrical Engineers of Japan
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