This paper reports on a microdevice for compartmentalization of solution microenvironment that can fractionate the space surrounding spheroids in the culture and differentiation processes, with the aim of verifying the effects of cell-cell interactions in solution microenvironment on iPS cell differentiation. In this paper, we demonstrated that spheroids of iPS cells were successfully formed on the device and fractionated their microenvironment by a detachable compartmentalization frame (DCF) formed by 3D printing technology.
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