抄録
Mycelium of Aspergillus niger was cultured aerobically in a liquid medium and immobilized on a carbon electrode. The electrode with immobilized Aspergillus niger was immersed in a buffer solution at pH 6.0 and the current at 0.5 V was measured. The electrode produced an anodic current with the addition of p-benzoquinone as an electron transfer mediator and attained a steady-state. When glucose was added to the solution containing p-benzoquinone, the current increased further and attained a steady-state. The steady-state current with glucose increased with increasing concentrations of glucose. Thus, Aspergillus niger immobilized on the electrode work as biocatalysts in the electrolytic oxidation of glucose. Characteristics and applications of the electrode were investigated.
