Influence of basic fibroblast growth factor in the solution and adsorbed form on the proliferation and differentiation of cells

抄録

There are some action modes to activate the receptor of growth factors and cytokines, such as juxtacrine, autocrine, and paracrine fashions. Some of growth factors transduce the signal to the target cell while anchored (immobilized or adsorbed) to a biological substance. For example, binding of fibroblast growth factor (FGF) to heparin is required for the signal transduction. Biological activity of growth factors, such as epidermal growth factor (EGF), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin, in the immobilized or absorbed form have been compared with that of factors in the water-soluble form. The growth factors immobilized or adsorbed maintain the activities to transduce the signals for activation of cell functions. The amount of growth factors immobilized to affect the proliferation of cells is smaller than that of water-soluble factors. There is difference in the time profile of the functional activation between the two forms of growth factor. This paper briefly reviews the influence of growth factors on the proliferation of cells while our data about the effect of basic FGF (bFGF) in different modes on the proliferation and differentiation of bone marrow derived stem cells.