抄録
Among human intestinal protozoa, only Giardia has not been yet cultivated. An attempt of the cultivation of Giardia muris was conducted by means of several artificial media. The following five different media were used in this experiment. 1. Balamuth's medium 2. Balamoth's medium added with thioglycollate and methionine 3. Asami's medium for Trichomonas Vaginalis 4. Asami's medium preconditioned with intestinal bacterial flora of mouse 5. Mixture of Asami's medium and Balamuth's medium, preconditioned as in the above. All the media were added with penicillin and streptomycin, in order to avoid the growth of bacteria that may be introduced with the inoculum. Giardia muris trophozoites in the intestinal tracts of mice were taken, as soon as the animals were sacrificed, and inoculated into these media and the media were kept at 37°C in a incubator.
The longest survival of the organism was observed in Asami's medium which was prepared to have the pH value of 5.0-5.2 and preconditioned with intestinal flora of the mouse. Sometimes, Giardias remained alive for 20 days in this medium, while in other media, they survived only for 8 days, at the longest. Though giardia did not show any evidence of multiplication, these findings seemed to have indicated that they require low redox potential.
The author also made an observation on the morphological differences between the cysts of Giardia lamblia and Giardia muris and came to the conclusion that cysts presented some reliable criteria for the differentiation of the species.
