抄録
After report No. 1 with the same experimental set, the author made the above titled experiments. In 15 adults dogs, the white cell count, white cell differentials Platelets count, coagulation time and bleeding times were measured. In 4 instances crystalloid solutions were used. In 5 instances glucose solution was used. And in 6 cases colloidal solution was used.
Results
White cell count decreased sharply in the experiments where crystalloid, glucose and glycoalgin solution was used for the replacement. While in the replacement experiments with dexstran and plasgen, this decrease was temporary and then returned to the pre-experimental value and finally increased, In the experiment with blood replacement, an initial dacrease was noticed but it was slight.
In all instances the differentials of WBC neutrophils and young cells increased while eosinophils, monocytes and lymphocytes decreased.
Erythroblasts were demonstrated in the instances where crystalloid, giycoalgin and blood were used for replacement.
Platelets were decreased in all instances.
Coagluation time wars decreased in the experiment with crystalloid replacement, In the case with glucose solution this decrease was also found but was a temporary phenomenon then it returned to the original value.
In the experiments with glycoalgin replacement, after the amount of blood depletion reached 40ml/kg dog's body weight, a prolongation of coagulation time was observed. In dextran and blood replaced dogs coagulation time was shortened.
Bleeding time was shortened in crystalloid replacement experiments after the removal of blood reached 10-20ml/kg, however, when the amount of blood reached 40ml/kg then the prolongation of bleeding time was noted.
In the glucose replacement experiments, when the blood loss reached 40ml/kg, there was shortening of the bleeding time, Mild prolongation was noted in glycoalgin replaced animals where bleeding amounted to 10-40ml/kg.
When blood was used for the purpose of replacement, bleeding time stayed normal until the withdrawn blood amount reached 60ml/kg when a prolongation of bleeding time started to appear.
