抄録
Prior to the measurement of sensitivity of tubercle bacilli against antituberculous agents, in sputum smear or through cultivation, by means of slide culture method, the author studied mainly Tsuji's Modification of Berry & Lowrry's method, and obtained the following improvements:
1) In case of slide culture of tubercle bacilli through cultivation, it is better to prepare 1 mg/ml of bacilli emulsion with 50 to 100% horse serum and to use slightly centrifuged supernatant part of the emulsion for the smear.
2) The optimal pH value of Kirchner's liquid medium for the slide culture method is around 6.8.
3) It is recommended to add 0, 001% of malachit green to the medium, in order to inhibit the growth of saprophyte.
4) The suitable number of culture days of tubercle bacilli in the said slide culture method is 7 days, in case of sputum, and 10 to 14 days in case of cultivation.
5) The count of more than 11 bacilli, in case of sputum, can be generally considered as of positive growth, and sometimes more than 21 bacilli, in case of cultivation.
