Chromosome Botany
Online ISSN : 1881-8285
Print ISSN : 1881-5936
ISSN-L : 1881-5936
Molecular phylogeny of Ochrosia sensu lato (Apocynaceae) based on ITS sequences data: An evidence for the inclusion of Neisosperma
HendrianKatsuhiko Kondo
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2007 年 2 巻 4 号 p. 127-132

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Molecular phylogeny of Ochrosia sensu lato (Ochrosia sensu stricto and Neisosperma) was investigated by maximum parsimony analysis of sequence data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA. Twenty-one species including two varieties (25 accessions), were defined as the ingroup. Three outgroup species were selected from tribe Vinceae (Kopsia arborea, K. flavida, and Rauvolfia serpentina). The ITS sequence data showed that Ochrosia s.l. is a highly defined monophyletic group (supported with 100% bootstrap value). As shown in the strict consensus tree, all Neisosperma species were placed at the basal part of the tree with N. glomerata and N. nakaiana as the basalmost taxa. All species belong to Ochrosia s.str. were grouped into one clade and nested within Neisosperma resulting in monophyletic Ochrosia s.str. and paraphyletic Neisosperma. This suggested that Neisosperma should be included into Ochrosia, and the two groups are treated as a single genus. The analysis also showed that subdivision of Ochrosia s.l. into sections based on fruit morphology (fibrous vs. massive and thick endocarp) is not supported.
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© 2007 International Society of Chromosome Botany
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