抄録
Genetic diversity of Satakentia liukiuensis in some cultivated individuals in Bogor Botanical Garden (Indonesia) and Nong Nooch Tropical Garden (Thailand) compared with natural population was analyzed by RAPD markers. Satakentia liukiuensis is a monotypic genus and an endemic palm species in Japan and it distributed in very restricted area in Ishigaki Island and Iriomote Island in Yaeyama Group of the Ryukyu Islands. The ten of RAPD primers (Operon Tech. Inc.) were used in RAPD analysis generated 82 amplifi ed bands, which is 27 bands (32.9%) were polymorphic. A phenogram generated by UPGMA clustering analysis shows all accessions from natural population were separated from cultivated individuals with genetic distance 0.104. Genetic variation among accessions in natural population was lower than cultivated individuals. The genetic uniformity of S. liukiuensis in natural population may due to the result of the inbred nature of its small population size for a long time. In our opinion, the existence of S. liukiuensis will be sustainable, since the natural population of the species in Ishigaki Island was established as protected areas and managed by Satake Corporation and also the corporation announced and built `The Toshihiko Satake Memorial Palm Museum` in natural locality sites of the species. RAPD method is a very fast way of obtaining genetic information and the methods require no prior knowledge of the DNA sequence to generate genetic markers.
