歯槽骨再生の定量測定法とエムドゲイン^(R)ゲルの歯槽骨再生誘導活性

抄録

To establish effective regenerative periodontal therapy, a two-dimensional quantitative assay for regenerated alveolar bone in rats was developed, and the regenerative activity of Emdogain^<(R)> (EMD) as well as collagen sponge (CLS) and acid-polyglycolic gelatin sponge (GEL) was evaluated. After anesthetization of a Wistar rat, alveolar bone defects were prepared at both palato-distal sides of the maxillary first molars. The biomaterial was topically applied to one defect, and the other quadrant defect without application was designated as a control. One to 8 weeks after application, the animals were sacrificed, and the new bone formation (NBF) on the bone samples was measured by a newly-developed two-dimensional scale. The NBFs were observed at Week 1 and then gradually increased in all sites. The NBF in EMD-applied sites at Week 1 was significantly lower, and those in GEL-applied sites at Weeks 4 and 5 were significantly higher than those in controls (all p<0.05). However, the regenerated bone densities assayed by X-ray micro-CT tomograms in EMD-applied sites were significantly higher than the control. The present findings indicate that the developed assay could be a useful method for evaluation of the regenerative activities of biomaterials, and that EMD could induce the regeneration of alveolar bone with relatively higher density.