Ethyl-α-_D-Glucoside Production in a Bioreactor Using Immobilized Aspergillus kawachii N-3

Abstract

　In this study, we screened several fungi for the production of α-ethyl glucoside (α-EG) and successfully identified Aspergillus kawachii N-3 that exhibited high activity for both α-glucosidase and amylase. High-yield production of α-EG from maltose or soluble starch was achieved at pH6 and 30℃ in the presence of 30% ethanol. Moreover, continuous α-EG production from a commercial syrup (Sun-Oligo: Sanmatsu Co., Ltd.) using A. kawachii N-3 immobilized on a non-woven polyester fabric was successfully accomplished for a period of ＞90 days.