Abstract
We developed a new method of the determination of human serum and urine amylase as a routine analysis for clinical purpose, using β-2, 4-dichlorophenylmaltopentaoside as substrate. The new method is based on the following reactions.β-2, 4-dichlorophenyl-G5human→α-amylase β-2, 4-dichlorophenyl-G2+G3β-2, 4-dichlorophenyl-G2+G3α-glucosidase→β-2, 4-dichlorophenyl-G1+4G1β-2, 4-dichlorophenyl-G1β-glucosidase→2, 4-dichlorophenol+G12, 4-dichlorophenol+4-aminoantipyrine KIO4→Red Quinone Dye (505 nm)In this method, the amylase activity in human serum and urine is accurately determined without interference from endogeneous glucose.
