Abstract
A gas-liquid chromatographic method was examined in order to determine kojibiose and maltose in their mixture. The two disaccharides proved to be determined by this method, in which they were estimated from the ratio between the heights of the peaks on the gas-liquid chromatogram of α-maltose and α-kojibiose. The amounts of disaccharides produced from soluble starch through the transglucosylation of buckwheat α-glucosidase were quantitatively determined for the first time by a combination of a gas-liquid and a paper chromatographic methods. In the initial stage of the transglucosylation of the α-glucosidase, kojibiose, nigerose and maltose were remarkably formed, and isomaltose was the minor product. However, isomaltose was gradually accumulated, and other disaccharides disappeared rapidly with the elapse of reaction time.
