Evaluation of ACTH assay based on chemiluminescence enzyme immunoassay and comparison of its reactivity with high-molecular-weight ACTH

Abstract

Tosoh Corporation developed a novel reagent to be used with AIA-CL automated immunoassay analyzers to quantify adrenocorticotropic hormone (ACTH) on the basis of the principles of chemiluminescence enzyme immunoassay. The coefficients of variation (CVs) for within-run and between-run reproducibilities ranged from 1.1 to 4.4%. They showed good linearity from 0 to 1,790 pg/mL, and the limit of quantitation was 1.650 pg/mL. With its high sensitivity, the assay may be useful for detecting the presence of ACTH-producing tumors that secrete high-molecular-weight ACTH. The measurement time of this method is shorter than that of the existing method, and it is considered highly useful for routine examinations.