抄録
The immobilization behavior of fibronectin onto plasma-surface modified titanium was evaluated by using the quartz crystal microbalance-dissipation (QCM-D) technique. The plasma-surface modifications included a thin-film coating of hexamethyldisiloxane (HMDSO) and an O2-plasma treatment on titanium. The contact angle of HMDSO-coatings against double-distilled water was 100 degrees, and dramatically decreased after the O2-plasma treatment with less than 5 degrees. XPS analysis indicated the introduction of an O2-functional group onto the HMDSO surface by the O2-plasma treatment. The QCM-D technique enabled evaluation the adsorption behavior of fibronectin. The largest amount of fibronectin adsorbed was on both as-coated HMDSO (hydrophobic surfaces) and O2-plasma treated at 10-min (hydrophilic surfaces) surfaces compared to O2-plasma treated (24-h) surfaces. These results indicated that two adsorption mechanisms of the fibronectin to substrates were considered, including hydrophobic interaction and ionic bond via OH group and/or O2-functional group concomitant with hydrophilic surfaces.
