Ozone nano bubble water (NBW3) seems to be suitable as an adjunct treatment to oral infectious diseases as for antimicrobial effects and compatibility with oral tissues to overcome disadvantage of conventional mouth rinses.The aim of this study was to determine the effect of NBW3 on sterilization and cytotoxicity. We analyzed the bactericidal effect of NBW3 against oral infectious bacteria. Cytotoxic ability was estimated using human gingival fibroblast cells stained with PI/Calcein-AM and fluorescence microscopy. The reduction in the number of bacteria in the NBW3 group was significantly greater than those in the CHX group and similar to that of Listerine® against P. gingivalis. Cytofluorometric assays revealed higher biocompatibility of NBW3 against fibroblasts.NBW3 had significantly bactericidal activity against oral infectious bacteria and biocompatible with mammalian cells compared with conventional mouth rinses. The oral application of NBW3 might have potential as an adjunctive treatment for oral infectious diseases.
