Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
ORIGINAL ARTICLES
Application of Porous Materials to Three-dimensional Tissue Culture in Capillary Formation in Vitro
Tomoharu OKAMURATetsunari NISHIKAWAMasahiro WATOKazuya TOMINAGAHirohito KATOKoichi IMAIAkio TANAKA
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2014 年 12 巻 2 号 p. 93-99

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The purpose of the present study is that attempting three-dimensional tissue culture of capillaries in vitro, using three kinds of porous scaffolds, coral, ceramic bone, and pumice stone. The proliferation of cells was enhanced time-dependently in the experimental group. 7, 14 and 21 days after addition of particles, the cell proliferation of the experimental group was significantly higher than that of the control group. In the surrounding region from the added pumice stone, capillary formation was low. Much cell proliferation and capillary formation was observed three-dimensionally around the coral and ceramic bone particles. In the coral additional group, the largest capillary width was observed in the anastomosis region of capillaries.

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© 2014 by Japanese Association of Regenerative Dentistry
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