血液・尿中での硫化物およびチオ硫酸塩の冷蔵保存変化

抄録

  The stability of sulfide and thiosulfate in human blood and urine during 4°C storage has been investigated. A human blood and urine samples supplemented with a known amount of sulfide or thiosulfate were stored at 4°C, and periodically analyzed for concentration of these compounds by GC-MS after pentafluorobenzyl derivatization. In urine supplemented with sulfide, its level was quickly decreased, resulting in no detection after 24 hr, and instead the metabolite thiosulfate was produced, which level reached the plateau of about 30% molar recovery of the added sulfide. In urine supplemented with thiosulfate, its level was quickly decreased within 1 hour and reached the plateau of about 50% molar recovery. In blood supplemented with sulfide, its level was quickly decreased within 1hr and the level reached the plateau of 60% molar recovery. The metabolite thiosulfate was produced, which level reached the plateau of about 50% molar recovery. Thiosulfate level in blood returned to the original level within a few hours after a quick decrease. The production of the derivatives of sulfide and thiosulfate in blood showed remarkable matrix effect (about one fifth in peak area) compared to those in water or urine. From blood supplemented with sulfide, a slightly raised recovery level of sulfide was obtained by addition of 2-mercaptoethanol, suggesting that a part of sulfide spiked to blood was trapped covalently in disulfide containing substances.