抄録
Universal immuno-peroxidase polymer (UIP) technique on the ABO blood grouping was applied to samples of 4 μm thick cross sections obtained from human scalp and pubic hairs by using a microtome.
The UIP technique consists of amino acid polymers which are conjugated to multiple molecules of peroxidase and anti-mouse Immunoglobulin that is reduced to its Fab' fragment. Advantages of the UIP technique are: a) more intense staining, b) skipping two steps of staining and c) lower background staining. This technique was carried out using monoclonal antibodies, mouse anti-A (diluted 1:10), anti-B (diluted 1:10) and anti-H as the primary antibodies. With this technique, specific staining was shown as dark brown precipitates within the medulla of hair samples, and depending on the staining, respective blood groups were able to be determined.
From this investigation, it seemed that the present technique was of practical use for the ABO blood grouping of a sample from human scalp and pubes hairs.
