抄録
Purpose of the present study is to develop of a simplified intravenous fat tolerance test. A single dose of fat emulsion (0.25ml of 10% Intralipid/kg of body weight) was injected intravenously through the catheter inserted into the antebrachial vein taking for 90 seconds and the blood samples were drawn at 5, 8, 11, 14, 17, 20, 23 and 29 minutes after the midpoint of the injection. The test was done on 28 normal, diabetic or hyperlipidaemic subjects after an overnight fasting and at supine position.
Lipoprotein fraction of d<0.95g/ml was separated with ultracentrifugation according to Hatch's method. Light scattering index of serum and the lipoprotein fraction of d<0.95g/ml were estimated with nephelometer (Nepherotic DN-2110, Kyoto Dai-ichi Kagaku Co., Ltd.) after dilution with physiological saline by 100 times or 48 time respectively.
Disappearance curves were exponential as shown in Fig. 2. Fractional removal rate of Intralipid (K2) of the serum and the lipoprotein fraction of d<0.95 g/ml were calculated by the method of least squares using logarithmic value of the light scattering index (Fig. 3).
The K2 values of the serum and the lipoprotein of d<0.95 g/ml showed good agreement in magnitude and range, and well correlated (r=0.855, p<0.001) as shown in Fig. 4. K2 obtained in the present study was apparently higher than that obtained from other fat tolerance tests using large amount of Intralipid. A hyperbolic relationship was found between K2 of the serum or the lipoprotein of d<0.95g/ml and fasting serum triglyceride concentration (Fig. 5). The inverse relatioship between K2 values and fasting serum triglyceride became linear when the datas were transfered to their logarithm (Fig. 6).
These results revealed that the simplified fat tolerance test with small amount of fat emulsion and short sampling time is valuable to obtain K2 value and a convenient tool to study pathogenesis of hypertriglyceridaemia.
