抄録
Rice yellow mottle virus (RYMV) is a plant pathogenic virus that often causes a serious damage to rice production in Africa. In this study, we developed detection systems of RYMV DNA or RNA using each of PCR, recombinase polymerase amplification (RPA), and an RNA-specific amplification. The sensitivities were in the range of several copies of the target DNA for PCR and RPA and dozens copies of the target RNA for RNA-specific amplification. The cycle numbers or reaction times required for amplification from 109 copies of the target DNA or RNA were 15 cycles (27 min) for the PCR-based system and 5 min for RPA- or RNA-specific amplification-based systems. These results suggested that isothermal RPA and RNA-specific amplification-based detection systems of RYMV will be more suitable for quick detection of RYMV-infected rice plants than the PCR-based one.
