抄録
We studied the anti-dyslipidemic effects of Petasites japonicus subsp. giganteus (PJG) in vitro and
in vivo. A water extract from the leaves of PJG (PJGL-WE) markedly inhibited lipoprotein production
from Caco-2 human colonic carcinoma cells differentiated into intestinal epithelium-like cells
and from HepG2 human hepatoma cells. We examined the effects of PJGL-WE on the expression of
lipogenic enzymes in both cells at the mRNA level. As a result, PJGL-WE suppressed gene expression
involved in transacylation, such as monoacylglycerol acyltransferase 2 and diacylglycerol acyltransferase 2; however, it did not affect enzymes involved in fatty acid synthesis. PJGL-WE also attenuated the expression of microsomal triglyceride transfer protein and apolipoprotein B100, elements required for lipoprotein synthesis. Furthermore, we studied the actions of PJGL-WE on adipose tissue accumulation, intrahepatic lipid content, and plasma parameters in vivo. PJGL-WE suppressed mesenteric adipose tissue accumulation and normalized intrahepatic triglyceride content and plasma triglyceride levels in mice fed a high-fat diet. These findings suggested that PJGL-WE attenuated the expression of genes required for lipid and lipoprotein synthesis, thereby suppressing lipid absorption from the small intestine and participating in the improvement of fatty liver and normalization of plasma triglyceride levels.
