Efficiency of Pheophorbide-Dimer in Photodynamic Therapy of Mouse Tumor
キーワード:
pheophorbide-dimer (Pheo-dimer),
photodynamic therapy (PDT),
pheophorbide a (Pheo),
FM3A tumor
1988 年 5 巻 2 号 p. 145-153
詳細
1988 年 5 巻 2 号 p. 145-153
We have investigated the efficiency of pheophorbide-dimer (Pheo-dimer) localization into tumors and the effect of photodynamic therapy (PDT) using Pheo-dimer on mice bearing FM3A tumors as a part of our search for new photosensitizers. Pheo-dimer was synthesized by covalently joining two molecules of pyropheophorbide a (Pyro) with an arginine as a cross-link. The in vitro photodynamic inactivation of FM3A cells by Pheo-dimer was less than that by pheophorbide a (Pheo). The FM3A tumor took up Pheo-dimer gradually as well as Pheo, but the time required for localization of Pheo-dimer was faster. The time for attaining the maximum concentration of Pheo in the tumor after injection was 18-24h, while that of Pheo-dimer was 9-12h. A small amount of Pheo-dimer was degraded to monomers by breakage of its cross-link in the body.
When irradiation was performed at 10h following administration of Pheo-dimer, the longer survival of mice bearing FM3A tumors was found in comparison with that obtained when Pheo was used. The utility of Pheo-dimer was thus expected in PDT.
